Regulatory

Part:BBa_K4119016

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)


PthlA-FNRbindingsite-7-withRBS

PthlAF7 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 7 bp from the - 35 region of the PthlA promoter.



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Results:

The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.

Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Parameters
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